dna molecular weight in g mol
The molar mass is defined as the mass of a given substance divided by the amount of a substance and is expressed in gmol. DsDNA ssDNA RNA olig Size of the nucl.
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660 grams per mole per base pair.
. Then chose the correct conversion factor. DNA molarity in mol. 260 nm extinction coefficient.
Converting mass of a DNA band into moles. 330 g mol b Double-stranded. For example one microgram µg 10-6 grams of DNA pieces 1000bp long is 152 picomoles pmol 10-12 moles and 1pmole of DNA pieces 1000bp long will weigh 066µg.
4870 dCTP 4672 dGTP 5072 dTTP 4822 dAMP 3312 Average. MW ssRNA N A xMW A N U xMW U N C xMW C N G xMW G 159. NEBioCalculator - using the ds mass-moles module to plan an NEBuilder HiFi DNA assembly reaction.
DNA Molecular Weight accepts one or more DNA sequences and calculates molecular weight. Molar Mass Molecular Weight and Elemental Composition Calculator Enter a chemical formula to calculate its molar mass and elemental composition. Of ssRNA eg RNA Transcript.
DNA molecular weight in gmol. 3270 dCMP 3072 dGMP 3472 dTMP 3222 Spectral Constants for Ribonucleotides Compound Molecular Weight gmol ATP 5072 Average. Molar mass of DNa is 25003871058 0000000020 gmol.
The additional 79 refers to the existence of a 5phosphate residue. In the following boxes always fill all but one field and click Calculate. Number of molecules in 1 μg.
The molar mass is usually the more appropriate figure when dealing with macroscopic weigh-able quantities of a substance. Molarity of 1 μgμl solution. A260 of 1 μgμl solution after 100-fold dilution.
Weight to Molar Quantity for nucleic acids This is used to convert the weight weight concentration into the molar quantity molar concentration for nucleic acids and vice versa. Single-stranded sequences are assumed to have a 5 phosphate. A protein whose molecule has an average mass of 64 kDa would have a molar mass of 64 kgmol.
M w MW. Sequences can be treated as double-stranded or single-stranded and as linear or circular. The molecular mass and relative molecular mass are distinct from but related to the molar mass.
Actively helping customers employees and the global community during the coronavirus SARS-CoV-2 outbreak. For example the average mass of one molecule of water is about 180153 daltons and one mole of water is about 180153 grams. Nucleic Acids Sizes and Molecular Weights Spectral Constants for Deoxyribonucleotides Compound Molecular Weight gmol dATP 4912 Average.
If only DNA length is given the molecular weight is calculated as. Mass of ssDNA g moles of ssDNA mol x length of ssDNA nt x 30897 gmolbp 1802 gmol. The molecular weight of A T C G equals to 3132 3042 2892 and 3292 gmol respectively.
It is often necessary to express amounts of DNA in terms of both weight and number of molecules. MW ssDNA N A xMW A N T xMW T N C xMW C N G xMW G 79. Double strand DNA base weight is 650 Dalton for single strand is 330 for RNA is 340 Dalton.
That is the molar mass of a chemical compound was meant to be numerically equal to its average molecular mass. First decide if the band is composed of single or double-stranded DNA. 330 grams per mole per base.
Molecules of DNA and 1 mole of bowling balls is 6023 x 1023 bowling balls. Molecular Weight to Molarity MW or Molar Mass of Solute gmol Desired Molarity molar M millimolar mM micromolar uM nanomolar nM picomolar pM. The molecular weight of A U C G equals to 3292 3062 3052 and 3452.
19 rows Nucleic Acid Molecular Weight Conversions. If the DNA is single-stranded then multiply the number of bases by the average mass of a single base 330 gmol to get the approximate mass of the whole molecule. Mass of dsDNA g moles of dsDNA mol x length of dsDNA bp x 61796 gmolbp 3604 gmol Videos.
DNA weight in g. MW DNA Length bp DNARNA base weight.
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